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KMID : 1161320190340040259
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Journal of Animal Reproduciton and Biotechnology
2019 Volume.34 No. 4 p.259 ~ p.266
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Principle of Emulsion PCR and Its Applications in Biotechnology
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Chai Chang-Hoon
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Abstract
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Emulsion polymerase chain reaction (PCR) is performed on compartmentalized DNA, allowing a large number of PCR reactions to be carried out in parallel. Emulsion PCR has unique advantages in DNA amplification. It can be applied in many molecular biological assays, especially those requiring highly sensitive and specific DNA amplification. This review discusses the principle of emulsion PCR and its applications in biotechnology. Related technologies are also discussed.
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KEYWORD
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DNA enrichment, droplet digital PCR, emulsion PCR, polymerase chain reaction, SELEX
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